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murine astrocyte cells  (ATCC)


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    ATCC murine astrocyte cells
    Murine Astrocyte Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+astrocytes/RAW+264%2E7/pm42312865-51-7-11
    Average 99 stars, based on 24399 article reviews
    murine astrocyte cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Coenzyme Q10 Protects Astrocytes from Ultraviolet B-Induced Damage Through Inhibition of ERK 1/2 Pathway Overexpression.
    Article Snippet: Overexpression of extracellular signal-regulated kinase 1⁄2 (ERK 1⁄2) signaling pathway leads to overproduction of reactive oxygen species (ROS) which induces oxidative stress.. Coenzyme Q10 (CoQ10) scavenges ROS and protects cells against oxidative stress.. The present study was designed to examine whether the protection of Coenzyme Q10 against oxidative damage in astrocytes is through regulating ERK 1/2 pathway.

    Modification:

    Article Title: β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes
    Article Snippet: .. Murine astrocytes (CRL-2541; ATCC, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle’s Medium (Gibco, Waltham, MA, USA) supplemented with 10% heatinactivated fetal bovine serum (Biological Industries, Beit-Haemek, Israel) and 100 U/mL penicillin–streptomycin (Gibco) at 37 ◦C under 5% CO2. .. The cells were seeded in 6-well plates (IWAKI, Shizuoka, Japan) for reverse transcription quantitative polymerase chain reaction (RT-qPCR) and monoamine analysis or in 10-cm plates (Corning, Corning, NY, USA) for MAO and COMT activity assays at a density of 3.0 × 105 cells/mL and incubated overnight. β-lactolin (GTWY peptide; purity: 98%) was purchased from Bachem (Bubendorf, Switzerland). β-lactolin was dissolved in dimethyl sulfoxide (Wako, Osaka, Japan) and diluted in phosphate-buffered saline (PBS; TaKaRa Bio, Shiga, Japan), such that the final concentration in the culture medium was less than 1%.

    In Vitro:

    Article Title: Long-Term Capsaicin Administration Ameliorates the Dysfunction and Astrogliosis of the Brain in Aged Mice with Missing Maxillary Molars.
    Article Snippet: .. Murine astrocytes from the C8-D1A cell line (ATCC® CRL-2541; Manassas, VA, USA) were used in in vitro experiments. .. The cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM L-glutamine (ATCC® 30-2002TM, Manassas, VA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Wako, 168-23191, Osaka, Japan).



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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice <t>cultures</t> showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) <t>Murine</t> cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced <t>astrocytes.</t>
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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice <t>cultures</t> showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) <t>Murine</t> cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced <t>astrocytes.</t>
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    murine astrocytes  (ATCC)
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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice <t>cultures</t> showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) <t>Murine</t> cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced <t>astrocytes.</t>
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    Image Search Results


    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice cultures showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) Murine cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced astrocytes.

    Journal: Cells

    Article Title: Targeting Astrocytic Connexin 43 Mitigates Glutamate-Driven Motor Neuron Stress in Late-Onset Spinal Muscular Atrophy

    doi: 10.3390/cells14231852

    Figure Lengend Snippet: Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice cultures showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) Murine cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced astrocytes.

    Article Snippet: A genetic knockdown was achieved by transfecting WT murine astrocyte cultures with mouse-specific SMN1 -siRNA (#SR408287, OriGene, Rockville, MD, USA) to induce SMN deficiency.

    Techniques: Glutamate Assay, Expressing, Control